银杏叶提取物对溶血磷脂酰胆碱引起的脑微血管细胞损伤的保护作用

Protective effect of Ginkgo biloba extract on the injury in cerebral microvascular cell by lysophosphatidylcholine

目的：研究银杏叶提取物（ＧｂＥ）对溶血磷脂酰胆碱（ＬＰＣ）引起的小牛脑微血管内皮细胞（ＢＣＭＥＣ）损伤及小牛脑微血管平滑肌细胞（ＢＣＭＳＭＣ）增殖的保护作用。方法：以乳酸脱氢酶（ＬＤＨ）释放量为指标测定ＢＣＭＥＣ损伤，用结晶紫染色法测定ＢＣＭＳＭＣ增殖。结果：ＬＰＣ５０ｎｍｏｌ／Ｌ可明显增加ＬＤＨ的释放，ＧｂＥ０，５，１０，２０，５０和１００ｍｇ／Ｌ，则ＬＤＨ释放率由４７．０％±０．７％分别下降为３９％±３％，３６．０％±１．３％，２７．０％±２．１％，１６％±６％和１３％±３％，表明ＧｂＥ呈剂量依赖性地抑制了ＬＤＨ的释放。ＧｂＥ对ＬＰＣ引起的ＢＣＭＳＭＣ的增殖抑制作用不明显。

AIM: To study the effects of protetion of Ginkgo biloba extract (GbE) on lactic dehydrogenase (LDH) release from bovine cerebral microvascular endothelial cells (BCMEC) and proliferation in bovine cerebral microvascular smooth muscle cells (BCMSMC) by lysophosphatidylcholine (LPC). METHODS: The injury of BCMEC was determined by the LDH release. The proliferation of BCMSMC was determined with crystal violet. RESULTS: GbE (5, 10, 20, 50 and 100 mg/L) inhibited LDH release by LPC (50 nmol/L) in a concentration_depen_ dent manner. The LDH release was reduced from 47.0%±0.7% (control) to 39%±3%, 36.0± 1.3%, 27.0±2.1%, 16±6%, 13±3%, respectively. GbE did not significantly antogonize the proliferation effect by LPC in cultured BCMSMC. CONCLUSION: GbE protects BCMEC injury by LPC.