钌配合物[Ru(bpy)2(PNT)]^2＋的合成、表征及与DNA相互作用研究

Synthesis,Characterization and DNA-Binding Studies of Ruthenium Complex [Ru(bpy)_2(PNT)]^(2+)

以cis-Ru(bpy)2Cl2.2H2O与PNT为原料合成钌(Ⅱ)多吡啶配合物[Ru(bpy)2(PNT)]2+(bpy=2,2′-联吡啶,PNT=2-[4′-(5-四唑基)苯基]咪唑-[4,5-f][1,10]邻菲咯啉),通过元素分析、质谱和核磁共振波谱对该化合物进行了结构表征.利用紫外-可见吸收光谱、荧光光谱、热变性和黏度实验研究了配合物与CT-DNA的相互作用,实验结果表明,该配合物以部分插入模式与DNA结合.

A novel ruthenium complex [Ru（bpy）2（PNT）]2＋（bpy=2,2＇-bipyridine,PNT=2-[4＇-（1H-tetrazol-5-yl）phenyl]-1H-imidazo[4,5-f][1,10]phenanthroline）was synthesized and characterized by elemental analysis,electrospray mass spectra and 1H NMR.The binding of the complexes with calf thymus DNA was investigated by absorption spectroscopy,luminescence titrations,viscosity measurements and DNA thermal denaturation experiments.The experimental results suggest that [Ru（bpy）2（PNT）]2＋ binds with DNA by partial intercalation.