肺癌GST-π异常表达及相关因素的数字图像处理分析

THE STUDY OF THE ABNORMAL EXPRESSION OF GLUTATHIONE S-TRANSFERASES ACID ISOZYME AND ITS CORRELATIVE FACTOR IN PATIENTS WITH LUNG CANCER BY USE OF DIGITAL IMAGE PROCESSING SYSTEM

为研究谷胱甘肽硫转移酶酸性同工酶（ＧＳＴ－π）在肺癌组织中的表达及相关因素，应用ＡＢＣ免疫组织化学方法对３７例肺癌组织ＧＳＴ－π表达进行测定，并采用数字图像处理进行分析。结果表明，ＧＳＴ－π在肺癌中有较高表达者占８３．８％（３１／３７），其中鳞癌９５．０％（１９／２０），腺癌７０．６％（１２／１７），且与腺癌分化有关，即分化程度愈高，表达愈高，灰度值愈低。肺癌中伴肺门或纵隔淋巴结转移者９５．８％（２３／２４）和吸烟者９６．３％（２６／２７）高表达，灰度值低。同时对３１例ＧＳＴ－π高表达患者进行化疗药物敏感性测定，其中１９例（６１．３％）对阿霉素或卡铂耐药。结果提示，ＧＳＴ－π可作为鳞癌和高分化腺癌的标志酶，也可作为判断预后和提示癌细胞产生耐药性的标志酶，测定数据用数字图像处理后科学性强。

Using ABC immunohistochemistry method and digital image processing system apparatus, we examined the expression of GST-π in patients with lung cancer, of 37 patients tested, 83.8% (31/37) showed high-expression.The positive staining in squamous cell carcinoma was 95% (19/20). In adenocarcinoma, the positive staining was 70.6% (12/17) and was correlated with its tumor cell differentiation, that is to say, the well tumor cell differentiated, the higher GST-π expressed, and the lower grey degree were. The expression of GST-π in lung cancer with hilar or mediastinal lymph node metastasis group 95% (23/24) and smoking group 96.3% (26/27) were high and showed low grey degree. In addition, we examined the susceptibility of 21 GST-π high expression patients to chemotherapeutic agents 19 (90.5%) patients showed drug resistant to Adriamycin or Carboplatin. It suggested that GST-π may be looked as not only the index enzyme of squamous cell carcinoma and high-differentiation adenocarcinoma, but also the index enzyme for prognosis and showing tumor cell production of drug resistant, digital image processing system is science.