桦褐孔菌菌粉多糖提取工艺的优化

Optimization of Extraction Technique of Polysaccharides from Fermentation Powder of Inonotus obliquus

根据Box-Benhnken的中心组合实验设计原理,在单因素试验的基础上采用三因素三水平的响应面分析法,以多糖提取率为响应值作响应面,并进行回归分析。结果表明,桦褐孔菌菌粉多糖提取的理想工艺条件为:温度为83℃,提取时间为2.2 h,液料比为33.3∶1时,桦褐孔菌菌粉多糖的提取率达到24.578%。气相色谱分析该工艺条件下提取的桦褐孔菌菌粉多糖,各主要单糖组成及质量分数分别为:阿拉伯单糖0.53%,甘露糖0.48%,葡萄糖10.75%,半乳糖2.44%。红外光谱分析结果显示,多糖产品中含有酸性多糖,糖苷键主要是α型。

The optimum conditions for the extraction of polysaccharides from fermentation powder of Inonotus obliquus were determined by using response surface methodology （RSM）. A three-level Box-Behnken factorial design was used to investigate the effects of three independent variables, namely extraction temperature, extraction time and liquid-solid ratio on the response, the yield of polysaccharides. The independent variables were coded at three levels and their actual values were selected on the basis of preliminary experimental results. Results showed that the generated regression models adequately explained the data variation and significantly represented the actual relationship between the independent variables and responses. An extraction temperature of 83℃, an extraction time of 2.2 h and a liquid-solid ratio of 33.3 were found to be optimal for polysaccharides extraction from fermentation powder of Inonotus obliquus. By means of additional experiments, the adequacy of this model was conformed. The monosaccharide components of polysaecharides extracted under optimum condition were arabinose 0.53%, mannose 0.48%, glucose 10. 75% and galactose 2.44%. Moreover, there were acidic polysaccharides in the products and the glycosidic bonds were α-types mainly.