微生物接种堆肥胡敏酸三维荧光特性研究

Study on Three-Dimensional Fluorescence Spectroscopy Characteristics of Humic Acid during Composting with Microbes Inoculation

为提高堆肥效率,试验采用接种微生物堆肥技术,通过对堆肥样品中胡敏酸三维荧光光谱特性进行动态分析,探讨了微生物接种对堆肥腐殖化进程的影响。在堆肥初期,胡敏酸只产生一个类富里酸荧光峰(peak a,Ex/Em为330/440)。在堆肥的336 h,与对照(RUNck)相比,接种微生物堆肥(RUNmic)胡敏酸三维荧光光谱中类富里酸特征峰(peak a,Ex/Em约为350/440)荧光强度明显减少,而类胡敏酸荧光峰(peak b,Ex/Em约为390/470)强度则略有增加;并且peak a和peak b对应的激发、发射波长均有一定程度的红移。胡敏酸元素分析表明,堆肥336 h,RUNck胡敏酸分子中C/H和O/C依次增加9.68%和10.91%;RUNmic中胡敏酸C/H和O/C依次增加了11.11%和20.37%,表明RUNmic中胡敏酸分子缩合度明显。以上结果证实,接种微生物可明加快堆肥腐殖化进程,进而提高堆肥效率。

Composting is one of the municipal solid wastes （MSW） treatment ways.In order to increase composting humification process,in the present study,inoculation microbes were used in MSW composting.The experiment design included RUNck with no microbes inoculation and RUNmic with microbes inoculation.The inoculation microbes were composed of Bacillus casei,Lactobacillus buchneri,Candida rugopelliculosa and Trichoderma.During composting,samples were taken from MSW composting,and humic acid（HA） was extracted from composting and purified,the three-dimensional-excitation emission matrix fluorescence spectroscopy（3DEEM） characteristics and elemental analysis of the HA were determined,and the effect of inoculation microbes on the degree of humification of composting was studied.At initial stage of MSW composting, HA 3DEEM exhibited a peak of fulivc-like at an Ex/Em wavelength pair of 330/440 nm（peak a）,and with MSW composting process,the excitation wavelength of peak a shifted from 330 to 350 nm.And a new peak of humic-like reformed nearby an Ex/Em wavelength pair of 390/470 nm（peak b）.At 336 h of MSW composting,compared with RUNck（with no micromial inoculation）,the fluorescence intensity of peak a（fulivc-like） nearby an Ex/Em wavelength pair of 350/440 in RUNmic（with microbial inoculation） was decreased,while that of peak b（humic-like） nearby an Ex/Em wavelength pair of 390/470 was slightly increased;the excitation and emission wavelength of peak a and peak b all shifted to long wavelengths.Elemental analysis indicated that C/H and O/C of HA molecule in RUNck increased by 9.68% and 10.91% respectively;and those of RUNmic increased by 1 1.11% and 20.37% at 336 h of MSW composting,respectively.The changes in HA 3DEEM and parameters of elemental analysis occurred in RUNmic at the final stage of composting,because degraded molecules,by the intense microbiological activity during composting,became reorganized to form more condensed compounds,richer in aromatic components.These results all led to the similar conclusion that inoculation microbes composting would accelerate the degree of HA humification,and increase the efficiency of composting.