反相高效液相色谱法测定中药菝葜中茋类成分的含量

Determination of stilbenoids in Smilax china L. by HPLC

目的:建立菝葜中茋类成分含量测定的RP-HPLC方法。方法:色谱柱为Zorbax SB C18柱(250mm×4.6 mm,5μm),流动相为乙腈-0.04%磷酸水溶液系统梯度洗脱,流速为1.0 mL.min-1,检测波长为320 nm,进样体积为10μL,柱温为30℃。结果:本方法可以准确测定菝葜中5个茋类成分:虎杖苷(polyda-tin),云杉鞣酚(piceatannol),氧化白藜芦醇(oxyresveratrol),白藜芦醇(resveratrol),三棱素A(scirpusin A)的含量。结论:本方法简便、准确、专属性强,可以实现对菝葜药材以及相关制剂质量全面、准确的控制和评价。

Objective：To develop a RP-HPLC method for the determination of stilbenoids in Smilax china L. Methods：The separation was carried out on the Zorhax SB-C1s column（250 mm ×4.6 mm,5μm） with a simple linear gradient of acetonitrile-0.04% phosphoric acid at a wavelength 320 nm. The column temperature was kept at 30 ℃. Results：The RP-HPLC method was usable for the quantification of five stilbenoids, polydatin, piceatannol, oxyresveratrol, resveratrol, scirpusin A. Conclusion： The method was simple, rapid and reproducible and could comprehensively control the quality of the drug of Smilax china L.