摘要
目的探讨骨髓源性树突状细胞（DC）对细胞因子诱导的杀伤细胞（CIK）增殖能力、免疫表型、以及抗淋巴瘤细胞的作用。方法取昆明小鼠骨髓单个核细胞在体外诱导DC和CIK细胞,同时用鼠脾脏单个核细胞诱导CIK细胞,将DC与CIK共培养,以CIK细胞单独培养为对照。用台盼蓝活细胞计数计算细胞扩增倍数,流式细胞术检测免疫表型,MTT法测定杀伤活性。结果DC-CIK细胞增殖能力明显高于CIK细胞（P〈0.05）,骨髓DC-CIK细胞与脾脏DC-CIK细胞增殖无明显差异。DC-CIK细胞共培养后,CD3^＋和CD3^＋CD8^＋、CD3^＋NK1.1^＋双阳性细胞比率较同条件下CIK细胞组显著增多（P〈0.05）;在5∶1-40∶1的效靶比范围内,DC-CIK细胞对淋巴瘤细胞的杀伤率显著高于CIK细胞（P〈0.05）,且杀伤率与效靶比呈正相关。结论骨髓源性DC增强CIK细胞的增殖能力,DC-CIK细胞增加抗淋巴瘤细胞的活性。
Objective To investigate proliferative capacity,immunophenotype,anti-tumor activity against lymphoma cells of DC-CIK cells in co-culture of cytokine-induced killer（CIK）cells with dendritic cells（DC）derived from bone marrow cells.Methods DC were prepared from Kunming mice bone marrow cells.CIK were induced from Kunming mice bone marrow cells and spleen mononuclear cells.DC-CIK was co-cultured peripherally.CIK cells were cultured alone as controls.Increased number of cells were counted by tapan-blue staining,cell immunophenotypes were analyzed by flow cytometry,killing activity was detected by MTT assay.Results The proliferation activity of DC-CIK cells was significantly higher than that of CIK cells（P〈0.05）.The DC-CIK cells from bone marrow or spleen showed no obvious difference in proliferation activity.Under the same condition,the ratio of CD3＋NK1.1＋,CD3＋CD8＋and CD3＋ double positive cells in CIK cells were significantly enhanced by co-culture with DC（P〈0.05）.At the effector-target ratio from 5∶1 to 40∶,the anti-lymphoma cells effect of DC-CIK cells was much higher than that of CIK cells（P〈0.05）,and this effect was positively related with the effector-target ratio.Conclusion The proliferation activity and anti-tumor effect of DC-CIK cells were significantly higher than those of CIK cells.Enhanced antitumour activities of CIK cells by co-culture with DC derived from bone marrow cells.The research provides experimental basis for the immunotherapy of DC-CIK cells.
出处
《实用临床医学（江西）》
CAS
2008年第12期4-7,共4页
Practical Clinical Medicine
基金
江西省教育厅基金(赣教技字[2006]75号)
