胶原酶对妊娠大鼠宫颈成熟的影响

Effect of Collagenase on the Cervical Riping of Pregnant Rats

目的研究胶原酶对妊娠大鼠宫颈成熟的影响。方法选取纯种wistar大鼠120只,其中雌鼠80只,雄鼠40只,按雌雄比例2∶1合笼饲养,共有64只雌鼠受孕,12只未孕,4只死亡。将64只妊娠大鼠随机分为实验组和对照组,每组32只,实验组大鼠在妊娠第17、18、19天08:00阴道内分别置入胶原酶栓1枚。2组大鼠均在妊娠第19天17:00乙醚麻醉下剖杀取宫颈组织,用高效液相色谱法测定2组宫颈组织羟脯氨酸的含量并观察宫颈病理变化。结果实验组的羟脯氨酸含量为(8.27±0.43)nmol/mg,对照组为(11.13±0.57)nmol/mg,2组比较差异有显著性(t=22.302,P<0.05)。结论胶原酶有较强的促宫颈成熟的作用。

Objective To investigate the effect of collagenase on the cervical riping of pregnant rats.Metholds There are 80 female rates and 40 male rates which were used in this study.The number of female rates were twice that of male rates in the cage.There were 64 pregnant rates,12 no pregnant,and 4 were dead.The 64 pregnant rates were divided stochastically into experimental group and control group with 32 samples in each group.Putting one collagenase suppository into the experimental group pregnant rates vagina at 8 clock in the morning of 17、18、19 pregnant day.The two groups rates were killed by aether narcosis to gain the cervical tissues at 5 clock in the afternoon of the nineteenth pregnant day.Hydroxyproline content of cervical uterine was measured by high resolution liquid chromatography,and the pathological changes of cervical uterine were observed.Results The Hydroxyproline of experimental group was（8.27±0.43）nmol/mg,but was（11.13±0.57）nmol/mg in the control group.There was significant difference between the two groups（t=22.30,P〈0.05）.Conclusion Collagenase could strongly promote the riping of cervical uterine.