体内构建组织工程腱鞘的初步研究

The Prime Study In Construction of Tissue Engineered Sheath In Vivo

目的研究体内构建组织工程化滑膜腱鞘,及其在预防肌腱粘连方面的作用。方法取Leghorn鸡48只,随机分为3组。实验组(n=16),切除肌腱外周滑膜腱鞘,采用自身滑膜细胞-PGA复合物修复缺损;对照组1(n=16),切除肌腱外周滑膜腱鞘后,采用空白PGA支架修复缺损;对照组2(n=16),切除肌腱外周滑膜腱鞘后未修复缺损腱鞘。于术后2周、5周取材,采用大体观察、组织学检查和生物力学测试等方法,研究组织工程腱鞘构建情况,分析肌腱表面粘连情况。结果大体观察及HE染色可见实验组形成组织工程化腱鞘,肌腱与腱周组织间空隙明显,较对照组粘连形成较少。力学检测在0.1N的拉力下,实验组肌腱拉伸位移3.24±0.22mm,优于对照组1和对照组2(分别为2.49±0.19mm,2.28±0.23mm)。结论采用组织工程技术可以成功构建组织工程化滑膜腱鞘,并具有正常腱鞘结构,对于减轻肌腱粘连具有一定作用。

Objectives To study the construction of tissue engineering sheath in vivo and its effects in preventing tendon adhesion. Methods Total 48 Leghorn chickens were randomly divided into three groups. Experimental group （n=16）： repairing the removed sheath with the synoviocytes-polyglycolic acid （PGA） complex; Control group 1 （n=16）： repairing the removed sheath using the PGA scaffold without cells. Control group 2 （n=16）： removing the nature tendon sheath without repaired. The chickens were sacrificed at the time point of the 2^nd, 5^th week for gross view and histological and bio-mechamical analysis. Results In the gross view and the HE staining, there was a tissue engineered sheath surround the tendon in the experimental group, and it formed less adhesions compared with the other two control groups. Bio-mechanically, the moving distance of the tendon in the experimental group was 3.24±0.22 mm. It was better than the control group 1 （2.49±0.19 mm） and the control group 2 （2.28±0.23 mm）. Conclusion It is feasible to construct the tissue engineering sheath using the tissue engineering technique, and it had the effect in preventing tendon adhesion.