摘要
为了揭示小麦(Triticum aestivum L.)品种间的遗传多样性,采用A—PAGE方法对58份来自不同育种单位的小麦新品种(系)进行了醇溶蛋白遗传多样性分析.全部供试品种(系)共检测到886条带,每份材料可电泳出8~20条带,平均15.3条.试验共获得迁移率不同的谱带86条,其中第2和第4条谱带出现的频率最高,分别为53.45%和50.00%,其余的谱带多态性很高,说明被检测的小麦新品种(系)间存在丰富的醇溶蛋白遗传异质性.供试材料间的遗传距离(genetic distance,GD)变异范围在0.26~0.83,平均为0.55.聚类分析在GD值为0.81水平上可将供试材料分为3大类群.研究数据为被检测材料在育种中的利用提供了有用信息.
The genetic diversity at gliadin loci of 58 new wheat cuhivars (lines) from different agricultural institutes was studied by A-PAGE. A total of 886 bands were identified in the examined wheat accessions. 8 to 20 gliadin bands were identified in each accession, with an average of 15.3 gliadin bands. A total of 86 bands of different relative mobility were observed by electrophoresis, among which No. 2 and No. 4 bands had the highest present frequencies of 53.45% and 50.00% respectively. The other bands showed very high polymorphisms. The result indicated that the new wheat cuhivars or lines examined in this study had a rich genetic diversity at the gliadin loci. The genetic distance (GD) of the tested accessions varied from 0.26 -0.83, with an average of 0.55. All of the tested materials could be classified into 3 clusters at the level of GD 0.81. This paper provides some useful information for wheat breeding with these wheat germplasms.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2008年第6期602-608,共7页
Journal of Henan Agricultural University
基金
河南省杰出人才创新基金项目(0621001700)
河南省科技厅攻关项目(0523010700)