参附注射液对心肺复苏大鼠心肌β肾上腺素受体活性的影响

Effects of Shen-fu on the activity of β-adrenoreceptors in cardiopulmonary resuscitation rats

目的探讨参附注射液对心肺复苏SD大鼠心肌细胞β肾上腺素受体活性的影响。方法实验在第二军医大学附属长征医院急救科实验室完成。SD雄性大鼠72只，随机分为9组（对照组；常规复苏0．5h，2h，4h，6h组；参附治疗0．5h，2h，4h，6h组），每组8只。采用窒息合并冰氯化钾（0．5mol／L）停跳液致大鼠心搏骤停心肺复苏模型，心搏骤停5min后开始心肺复苏，参附治疗组在心肺复苏的同时输入参附注射液（20mL/kg）。用1H-DHA放射性配基法测定心肌细胞8肾上腺素受体活性。所得数据以均数±标准差（x±S）表示，多组间比较采用单因素方差分析，两组间比较采用q检验。结果大鼠心肌细胞8。肾上腺素受体活性在心肺复苏后0．5h达峰值（247．81±8．91）fmol／mg，4h后下降至最低水平（104．17±13．87）fmol／mg，6h基本恢复正常（178．53±10．42）fmol／mg，参附组大鼠心肌细胞β肾上腺素受体活性在早期[0．5h：25（9．91±18．09）fmol／mg]与对照组差异无统计学意义（P〉0．05），而在后期[2h：（173．04±18．77）fmol／mg，4h：（148．72±13．88）fmol／mg]能显著提高大鼠心肌细胞口肾上腺素受体活性（P〈0．05）。结论大鼠心肺复苏后心肌细胞B肾上腺素受体活性经历先升高后降低的变化规律，参附注射液能够显著提高大鼠心怖复苏后期心肌细胞8肾上腺素受体活性。

Objective To study the effects of Shen-fu injection on the activity of β-adrenoreceptor in SD rats with cardiopulmonary resuscitation. Method The expriment was done at emergency medical lab of Shanghai Changzheng hospital. Cardiac arrest was induced through asphyxiation（succinylcholine） and ice-cold 0.5 mol/L KCl in rats,and resuscitation began at five minutes after cardiac arrest. In this experiment, seventy-two male SD rats were randomizly divided into 9 groups： control group （sham group）, routine treatment （0.5 h, 2 h,4 h,6 h） groups, and Shen-fu treatment（0.5 h,2 h,4 h,6 h） ,and each group had 8 rats. Rats of Shen-fu treatment groups were injected with shen-fu （20 mL/kg,20 mL/h） during CPR.3H-prazosln was used to determine the activity of adrenoreceptor in myocyte. The data of the statistic analysis were presented in the form of mean ± standard （ x ± s ） deviation. One-way ANOVA was used for raultigroup comparison, and t-test for comparison between two groups. Results β-adrenoreceptor reached peak at 0.5 h after CPR（247.81 ± 8.91 ） fmol/mg, reached the lowest level at 4 h（ 104.17 ± 13.87） fmol/mg, and retumext to the normal level at 6 h（ 178.53 ± 10.42） fmol/mg. In the early period,β-adrenoreceptor activity in control group [0.5 h： （259.91 ± 18.09） fmoL/mg] was similar to that in Shenfu treatment groups （P 〉 0.05）. In the late phase, β-adrenoreceptor activity in the Shen-fu treatment groups[2 h： （173.04 ± 18.77） finol/mg, 4 h：（ 148.72 ± 13.88） fmol/mg] was significantly higher than that in the control groups（ P 〈 0.05）. Conclusions After CPR, β-adrenoreceptor activity firstly rises and then declines, and Shen- fu injection can significantly increase β-adrenoreceptor activity of rats in the later period after CPR.