硫酸酯化箬叶多糖的结构修饰及其抗艾滋病病毒活性

SULFATION OF POLYSACCHARIDES ISOLATED FROMINDOCALAMUS TESSELATUS AND THEIR ANTICYTOPATHICEFFECT ON HUMAN IMMUNODEFICIENCY VIRUS TYPE I

从箬叶中分离纯化得到两种箬叶多糖，采用氯磺酸吡啶作为硫酸酯化试剂，研究反应温度、时间及反应物的摩尔比对硫酸酯化过程的影响。得到产率８０％以上，硫含量最高达２１９％的硫酸酯化多糖。发现硫酸酯化会使多糖构象发生一定的改变，硫酸酯多糖在特性粘度、比旋光度、园二色谱等性质方面有不同程度的变化。体外抗艾滋病病毒（ＨＩＶ）实验表明硫酸酯化箬叶多糖比箬叶多糖具有更高的抑制ＨＩＶ引起的ＭＴ４细胞病变性的作用，其最低有效浓度达５～１０μｇ·ｍｌ－１，细胞毒性浓度为２５ｍｇ·ｍｌ－１。

For preparation of sulfated polysaccharides isolated from bamboo leaves(Indocalamus tesselatus) with chlorosulfonic acidpyridine, the experimental conditions as the molar ratio of reactants and temperature and time of reaction were discussed. A homogeneous sulfation method with good yield of more than 80% was obtained and the sufur content was found to be 219%. Conformation change in sulfated polysaccharide with changes of intrinsic viscosity, polarimetery and CD spectra were found to be related to the negative charge. The polysaccharide sulfates were shown to have better activity to inhibit cytopathic effect of human immunodeficiency virus(HIV) infection than native polysaccharides. The effective concentration was 10 μg·ml-1 while the cytotoxic concentration was 25 mg·ml-1.