摘要
The allotetraploid in cross-fertilized eggs of Chlamys farreri♀× Patinopecten yessoensis♂ was induced in this paper.In order to find out the appropriate duration time of 6-DMAP treatment,ploidy status of induction offspring was analyzed by flow cytometry.The results showed that the optimal duration time of 6-DMAP treatment was to treat the fertilized eggs with 50 mg/L 6-DMAP for 15 min based on the integrated evaluation of the results of fertilization rate,survival rate of D-larvae,development status of larvae,and percentage of tetraploid.The results of flow cytometry showed that 17.3% tetraploid was obtained which showed that allotetraploid could be gotten with this method.The causes of the problems in the work were also discussed in this paper.
The allotetraploid in cross-fertilized eggs of Chlamys farreri ♀ × Patinopecten yessoensis ♂ was induced in this paper. In order to find out the appropriate duration time of 6-DMAP treatment, ploidy status of induction offspring was analyzed by flow cytometry. The results showed that the optimal duration time of 6-DMAP treatment was to treat the fertilized eggs with 50 mg/L 6-DMAP for 15 rain based on the integrated evaluation of the results of fertilization rate, survival rate of D-larvae, development status of larvae, and percentage of tetraploid. The results of flow cytometry showed that 17.3% tetraploid was obtained which showed that allotetraploid could be gotten with this method. The causes of the problems in the work were also discussed in this paper.
出处
《水产学报》
CAS
CSCD
北大核心
2009年第1期165-170,共6页
Journal of Fisheries of China
基金
国家科技支撑计划专题项目(2006BAD01A00)
国家“八六三”高技术研究发展计划(2006AA10A408)
国家自然科学基金项目(30600465)
关键词
栉孔扇贝
虾夷扇贝
异源四倍体
流式细胞术
Chlamys farreri
Patinopecten yessoensis
allotetraploid
flow cytometry（FCM）
