中药蒲黄黄酮成分高效液相色谱测定及质量评价

DETERMINATION OF FLAVONOIDS AND QUALITY EVALUATIONOF CHINESE TRADITIONAL DRUG “PUHUANG”

对中药蒲黄属（Ｔｙｐｈａ）的４种原植物花粉及部分种类的花丝、雌花和叶子中的５种黄酮单体成分，进行高效液相色谱测定［μＢｏｎｄｐａｋＣ１８柱，水—异丙醇—四氢呋喃（８３５∶１４∶２５）作流动相，检测波长２８７ｎｍ］，对其质量作了初步比较，并对蒲黄的质量标准提出初步建议。

This paper deals with the determination of flavonoids and quality evaluation of Chinese Typha. Four species, viz. Typha angustata, T.angustifolia, T.davidiana and T.latifolia and eight commercial samples of Chinese traditional drug “Puhuang” were collected. Five flavonoids in pollen, filaments, female flowers and leaves were determined by HPLC using μBondapak C18 column, water—isopropanol—tetrahydrofuran=835∶14∶25, as mokile phase and detected at 287 nm). The qualities of different Typha species and commercial “Puhuang” samples have been compared, and the criteria for their quality control were also discussed.