白三烯类化合物(Leukotrienes)对小鼠腹腔巨噬细胞生成白细胞介素6的影响

EFFECTS OF LEUKOTRIENES ON PRODUCTION OFINTERLEUKIN 6 FROM MOUSE PERITONEAL MACROPHAGES

探讨了白三烯Ｂ４（ＬＴＢ４）、白三烯Ｃ４（ＬＴＣ４）及白三烯Ｄ４（ＬＴＤ４）对小鼠腹腔巨噬细胞分泌白细胞介素６（ｉｎｔｅｒｌｅｕｋｉｎ６，ＩＬ６）的影响。用ＩＬ６依赖细胞株Ｂ９的ＭＴＴ方法测定样品中ＩＬ６的含量。结果显示，ＬＴＢ４能剂量依赖性地增加巨噬细胞培养上清液中ＩＬ６的含量。ＬＴＣ４及ＬＴＤ４促进巨噬细胞培养上清液中ＩＬ６含量的最适浓度分别为：６９×１０－８和８０５×１０－８ｍｏｌ·Ｌ－１。结果提示：ＬＴＢ４与ＬＴＣ４及ＬＴＤ４在某些生物学功能方面有一致性。与ＬＴＣ４及ＬＴＤ４相比，ＬＴＢ４促进巨噬细胞培养上清液中ＩＬ６的含量的最适浓度则要大１～２个数量级，提示在炎症反应中，肽白三烯与ＩＬ６的相关性较强。

In the present study, effects of leukotrienes on IL6 production by mouse resident peritoneal macrophages were explored with a bioassay method involving IL6 dependent murine hybridoma B9 cell line. The results showed that LTB4, LTC4 and LTD4 can enhance the production of IL6 from cultured peritoneal macrophages of mice. The optimal concentractions for LTB4, LTC4 and LTD4 were found to be 145×10-6, 69×10-8 and 805×10-8 mol·L-1, respectively. The results suggest that peptide leukotrienes might play important role on IL6 production in the local milieu of inflammation.