肿瘤细胞裂解物致敏的树突状细胞对结肠癌LoVo细胞的杀伤作用

Anti-Colon Cancer Immunity of Whole Cell Lysates Pulsed Dendritic Cell

目的探讨肿瘤细胞裂解物致敏的树突状细胞(DC)作为佐剂对结肠癌LoVo细胞系的杀伤作用。方法反复冻融法裂解培养的结肠癌LoVo细胞,提取细胞抗原并致敏DC,然后将后者与自身T淋巴细胞共同培养,观察其对自身T淋巴细胞增殖的影响以及活化的T淋巴细胞对LoVo细胞的杀伤作用。结果结肠癌LoVo细胞裂解物致敏的DC能明显促进T淋巴细胞的增殖,后者对LoVo细胞的杀伤作用明显增强。结论肿瘤细胞裂解物提取方法简单,易于临床实施,其作为细胞抗原致敏DC制备的肿瘤疫苗能有效活化并产生肿瘤特异性细胞毒T淋巴细胞,将有很好的临床应用前景。

Objective To detect the anti colon cancer ability of whole cell lysates pulsed dendritic cell （DC） which acts as an adjuvant. Methods Whole cell lysates of LoVo cells were extracted with freeze thawing method, then the monocyte-derived DC were pulsed with this cellular antigen. Subsequently, the capability of antigen pulsed DC to promote T lymphocytes proliferation and the ability of T lymphoeytes to kill LoVo cells were detected by 3 H-TdR incorporation and lactate dehydrogenase release methods, respectively. Results The whole cell lysates of LoVo cells pulsed DC significantly stimulated T cells proliferation, and the cytotoxicity abilities of primed T cells to kill LoVo cells were also enhanced. Conclusion Tumor cell lysates which act as the cellular antigen to pulse DC can improve the efficacy of anti cancer immune response, which provides us an experimental evidence for cancer immunotberapy.