毛细管电泳和聚丙烯酰胺电泳法检测肺癌组织p53基因突变效果分析

Detection of p53 gene point mutation in lung cancer tissue by capillary electrophoresis and polyacrylamide gel electrophoresis

目的筛选检测肺癌组织p53基因突变的优良方法,为临床早期诊断肺癌提供依据。方法分别运用毛细管电泳(CE)与聚丙烯酰胺电泳(PAGE)—限制性片段长度多态性(RFLP)两种方法对38例肺癌及癌旁正常组织p53基因第249位密码子点突变进行检测。结果RFLP-CE可检测到p53基因第249位密码子的野生型26 bp片段,用时<20 m in;RFLP-CE和RFLP-PAGE均能检测到基因突变,对29例非小细胞肺癌组织p53基因第249位密码子检测的突变率分别为31.03%(9/29)、20.69%(6/29),P<0.05。结论RFLP-CE可为大规模进行肺癌的早期诊断提供简便可靠的方法。

Objective To select the optimal method for the dection of p53 gene point mutation in lung cancer tissue. Methods 249 coden of p53 gene point mutation in lung cancer and normal lung tissue were detected by polyacrylamide gel electrophoresis （PAGE） and capillary electrophoresis （CE）respectively. Results The CE and PAGE could both detect the 249 coden of p53 gene point mutation. The detection time of CE was shorter, and the detection rate was higher. Conclusion CE can offer a convenient and reliable method to the large scale of early diagnosis of lung cancer.