三氧化二砷对神经母细胞瘤细胞增殖的影响

The effect of arsenic trioxide on the proliferation of neuroblastoma cells

目的探讨三氧化二砷（Ａｓ２Ｏ３）对儿童神经母细胞瘤有无潜在的治疗价值及伍用细胞因子有无增强疗效的作用。方法应用四甲基偶氮唑蓝法于体外观察Ａｓ２Ｏ３对神经母细胞瘤细胞株ＳＪ－Ｎ－ＳＨ增殖的影响；并观察Ａｓ２Ｏ３伍用重组人γ－干扰素、肿瘤坏死因子、白细胞介素４、６、１０后的情况。结果０．５～４μｍｏｌ／Ｌ的三氧化二砷明显抑制ＳＪ－Ｎ－ＳＨ细胞的增殖，Ａｓ２Ｏ３伍用γ－干扰素、白细胞介素４可明显增强Ａｓ２Ｏ３对ＳＪ－Ｎ－ＳＨ细胞增殖的抑制，而其他几种细胞因子无明显作用。结论Ａｓ２Ｏ３为治疗神经母细胞瘤的有效药物，联合应用合适的细胞因子可增强Ａｓ２Ｏ３的疗效。

Objective To probe into the potential therapeutic effect of arsenic trioxide (As 2O 3) on neuroblastoma.Methods The effect of As 2O 3 on the proliferation of neuroblastoma cell line SJ N SH was studied with MTT method in vitro. Results 0.5～4.0 μmol/L of As 2O 3 could significantly inhibited proliferation of SJ N SH cell. The inhibitory effect was augmented by adding interferon with inteulenkin 4.Conclusion These results suggested that As 2O 3 be an effective drug for neuroblastoma. Its therapeutic effectiveness could be augmented when the cytokines were added.