LC-APCI-M/SMS测定全血中苯妥英钠的含量

Determination of phenytoin sodium in blood by LC-APCI-MS/MS

目的：建立了LC-APCI-MS/MS法测定全血中苯妥英钠的血药浓度。方法：采用Zorbax Eclipse XDB-C18（4.6×150 mm,5μm）色谱柱,流动相为乙腈-0.1%甲酸溶液（50：50）。大气压喷雾离子源（APCI）,正离子检测,采用多反应监测方式进行定量分析,检测离子为m/z 253→182（苯妥英钠）,m/z 316→270（内标氯硝西泮）。结果：全血中苯妥英钠浓度在4～2400 ng/ml范围内线性关系良好,最低检测限为2 ng/ml。苯妥英钠的萃取平均回收率为71.45%～87.27%,日内及日间RSD都低于6%。结论：该法操作简便、快速、灵敏度高,可用于苯妥英钠的临床毒物分析。

Objective：To establish a LC-APCI-MS/MS method for determination of phenytoin sodium in blood.Methods：A Zorbax Eclipse XDB-C18（4.6×150 mm,5 μm） column was used,with mobile phase of acetonitrile-0.1% formic acid（50：50）.Atmospheric pressure chemical ionization（APCI）source was applied and operated in positive ion mode.Multiple reaction monitoring（MRM）mode was used.The detected ions were m/z 253→182（phenytoin sodium）,m/z 316→270（internal standard,clonazepam）.Results：The calibration curve of phenytoin sodium was linear in the concentration range of 4~2400 ng/ml.The LOD was 2 ng/ml.The average extraction recovery was 71.45%~87.27%.The RSDs of inter-day and intra-day were less than 6%.Conclusion：The analytical method for phenytoin sodium is accurate,sensitive and suitable for clinical toxicological analysis.