广东国境口岸中央空调水嗜肺军团菌检测及结果分析

Detection of Legionella from central air conditioning at frontier port of Guangdong

目的:为了解广东地区国境口岸中央空调冷凝水中军团菌的阳性率,分析军团菌对出入境旅客健康的潜在危害并为北京奥运会期间国境口岸军团菌防控提供理论依据。方法:通过对2007年度和2008年度广东地区6个国境口岸中央空调冷凝水的采集、处理,利用分离培养、乳胶凝集试验、血清学分型等方法对样本进行军团菌的检测及血清学分型,检验依据为ISO11731:1998。对检测结果进行分析。结果:2007年度从63份样本中检出军团菌7例,检测阳性率为11.1%,其中嗜肺军团菌4例;2008年度从46份样本中检出军团菌3例,检测阳性率为6.5%,全部为嗜肺军团菌。结论:本次军团菌检出率略低于有关文献报道的全国平均检出率,阳性样本中嗜肺军团菌L1型占比率较大(30%);本研究结果有利于分析国境口岸空调通风系统的卫生状况,为口岸卫生控制提供技术指导,有利于北京奥运会和谐、顺利举办。

Objective：To find out the positive ratio of strain in the genus Legionella from central air conditioning at the frontier port of Guangdong and to analyse its potential harm to the people at the port.Methods：The samples were collected from the condensate water of central air conditioning in 2007 and 2008 at the frontier port of Guangdong province.Legionella was isolated and identified based on the ISO 11731： 1998.Results：Seven strains of Legionella were obtained from 63 samples in 2007 and the positive ratio was 11.1%.Four of them were Legionella pneumophila.Three strains of Legionella pneumophila were isolated from 46 samples in 2008 and the positive ratio was 6.5%.Conclusion：The positive ratio of Legionella in this study is lower than average level of our country according to correlative literature report.The 30% of the positive samples are allocated to Lp1 genotype.