不同血细胞检测系统血常规测定结果的可比性分析

Comparative study on result of blood cells with different hematological analyzers

目的:通过不同检测系统对血常规测定结果进行方法比对,探讨不同血细胞分析系统对同一标本其测定结果的可比性。方法:按照NCCLS的EP9-A2文件要求,以Sysm ex XE-2100血细胞分析仪为目标检测系统(对比方法),Sys-m ex KX-21、CD-1700、BC-3000为实验检测系统(实验方法)。用病人新鲜全血对WBC、RBC、HGB、PLT进行检测,计算相关系数和回归方程,以美国临床试验室修正法规(CLIA,88)规定的室间质量评价允许误差范围的1/2为标准,判断不同检测系统测定结果的可比性。结果:除检测系统1(Sysm ex KX-21血细胞分析仪)的PLT低值测定结果、检测系统2(雅培CD-1700血细胞分析仪)的WBC低值的测定结果外,其余项目在不同检测系统上的测定结果在比较范围与检测系统4(Sysm ex XE-2100血细胞分析仪)均具有可比性。结论:应定期对不同血细胞检测系统进行测定结果的可比性分析,以及时发现系统误差,并进行校准,以确保检验结果的准确可靠。

Objective：To investigate comparability of blood cells results with different hematological analyzers.Methods：Following NCCLS document EP9-A2,detectings system 4（comparison method,Sysmex XE-2100）and detecting system1-3（laboratory method,Sysmex KX-21,CD-1700,BC-3000） were used to detect 4 items including WBC,RBC,HGB,PLT and to obtain the correlation coefficient and the linear equation.Evaluate the system bias between laboratory method and comparison method according to the half of CLLA.Results：ALL results could be accepted except the bias of PLT of system 1 and WBC of system 2 at lower value.Conclusion：It is necessary to do method comparison to judge clinical acceptability with different hematological analyzers.