子宫颈癌及癌前病变单纯疱疹病毒2型Ki-67的表达

A study on the HSV-2 infection and Ki-67 expression of the cervical carcinoma and cervical intraepithelial neoplasia

目的研究子宫颈癌及癌前病变中单纯疱疹病毒2型（HSV-2）感染和增殖细胞核抗原Ki-67的表达情况。方法采用PCR技术及免疫组织化学检测84例子宫颈癌组织（鳞状细胞癌67例、鳞腺癌3例、腺癌14例）、35例宫颈上皮内瘤变（CN）组织、13例正常宫颈组织的HSV-2、Ki-67的表达情况。结果（1）HSV-2阳性率为40．2％（53／132），其中HSV-2阳性率在子宫颈癌组织中为48．8％（41／84），在CN组织中为28．5％（10／35），在正常宫颈黏膜上皮中为15．4％（2／13）。癌组织、癌前病变HSV-2DNA表达明显高于正常宫颈组织（P〈0．01）。（2）Ki-67的阳性细胞在正常子宫颈、CN和子宫颈癌组织中表达逐级增加，显示鼬．67表达程度与组织学类型有关。HSV-2和Ki-67在CN和癌组织中的表达呈显著相关（P〈0．01）。结论宫颈癌及癌前病变组织中存在HSV-2感染，HSV-2感染和Ki-67表达率密切相关，两者的共同作用可能是宫颈癌发生、发展的重要因素。

Objective To explore the herpes virus Ⅱ （HSV-2） infection and Ki-67 expression for the cervical carcinoma and cervical intraepitbelial neoplasia（CN）. Methods The polymerase chain reaction（PCR） and immunohistochemistry were applied to detect the HSV-2 and Ki-67 expressions in cancerous tissues of uterine cervix in 84 cases（67 scaly cell carcinoma,3 scaly adenoearcinoma, and 14 adenocaxeinoma cases） , CN in 35 eases, and normal cervical tissues in 13 cases. Results （ 1 ）The positive rate of HSV-2 is 40.2% （53/132） , and the rate in cervical cancerous tissues, CN and normal cervical tissues were 48.8% （41/48） ,28.5% （10/35） and 15.4% （2/13） respectively. HSV-2 DNA expressions in cancerous tissue and CN were much higher than that of normal cervical tissues （P 〈 0.01 ）. （2）The Ki-67 positive cells were progressive increasing in the normal cervical tissues, CN and cervical cancerous tissues ,thus showing that there were relations between the Ki-67 expression degree and tissue types. The HSV-2 and Ki-67 expressions were remarkably connected（ P 〈0.01 ）. Conclusion There are HSV-2 infection,close related between HSV-2 infection and Ki-67 expression in the tissues of cervical carcinoma and CN. And the cooperation of them may play an important role in the development of cervical carcinoma.