清热暗疮片中大黄素与大黄酚的含量测定

Determination the Contents of Emodin and Chrysophanol in Qingreanchuang Tablet

目的:建立清热暗疮片中大黄素与大黄酚的含量测定方法。方法:采用HPLC法进行含量测定,以十八烷基硅烷键合硅胶为填充剂,甲醇-0.1%磷酸溶液(85:15)为流动相,检测波长254nm,流速为1.0ml·min^(-1)。结果:大黄素在0.0112～0.0560μg,大黄酚在0.0224～0.1120μg范围内峰面积与进样量有良好的线性关系。大黄素的平均回收率为98.6%,RSD为1.17%,大黄酚的平均回收率为98.9%,RSD为1.02%。结论:所建立的方法可以有效地控制制剂的质量。可准确、快速地进行定性、定量检测。

Objective： To establish the method for determination the contents of Emodin and Chrysophanol in Qingreanchang tablets. Method： The contents of Emodin and Chrysophanol were determined by HLPC. The stationary phase was kromasil C18 column. The mobile phase was methanol - 0. 1% phosphoric acid （ 85： 15 ）. The detective wave was 254 mn. The flow rate was 1.0 ml· min - 1. Result ： The HPLC method was reliable as the average recovery was 98.6% for Emodin with RSD 1.17% and 98.9% for Chrysophanol with RSD 1.02% respetively. The linear relationship of Emodin and Chrysophanol were between 0. 0112μg-0. 05600,g and 0. 0224μg -1. 1.1120μg respetively. Conclusion： The method is simple and accurate, and can be effectively controlled the quality of Qingreanchang tablets.