高效液相色谱法测定子宫锭中儿茶素的含量

Determination the Catechin in Uterus Lozenge by HPLC

目的:建立子宫锭中儿茶素的含量测定方法。方法:色谱柱为Agilent HC C_(18)柱(250mm×4.6mm,5μm),流动相为0.04mol·L^(-1)枸橼酸溶液-N,N-二甲基甲酰胺-四氢呋喃(THF)(45:8:2),检测波长280nm,柱温35℃,流速1.0ml·min^(-1)。结果:儿茶素在501.2～1754.2ng之间呈良好的线性关系。平均加样回收率为98.47%,RSD=1.92%(n=9)。结论:方法简便可靠,分离度好,可用于子宫锭的质量控制。

Objective： To establish a method for determination the content of catechin in Uterus Lozenge, Method： Agilent HC C18 column （ 250 mm × 4.6 mm, 5 μm） was used,0.04 mol· L^-1 citric acid-N, N-dimthylformamide-THF （45： 8： 2 ） was used as mobile phase. The UV detector wavelangth was 285 nm, column temperature was at 35 ℃ and the flow rate was 1.0 ml· min^-1. Result： The linear range of cateehin was 501.2 - 1754.2 ng. The average recovery was 98.47% ,RSD = 1.92% （ n = 9 ）. Conclusion： The method is simple, reliable, with a good separation, so it can be used for the quality control of Uterus Lozenge.