大花蕙兰腋芽增殖体系的建立

Establishment of Axillary Buds Regeneration System of Cymbidium hybridum

[目的]建立大花蕙兰腋芽增殖体系。[方法]以大花蕙兰的嫩芽为试材，研究大花蕙兰腋芽增殖的最佳条件。[结果]结果表明，腋芽增值的最佳培养基为B5＋6-BA3.0～4.0mg/L＋KT0.5mg/L＋NAA 0.1mg/L＋AD 2.0mg/L＋YE 100.0mg/L；生根阶段培养基为1/2MS＋2.0g/LAC＋0.3mg/L NAA生根效果好,生根率为90%，根数3～4条,6-BA添加与否影响不大。[结论]该试验通过腋芽增殖模式，遗传稳定性较高。

[ Objective ] The purpose was to establish regeneration system of Cymbidium hybridum axillary buds. [ Method ] With axillary buds of Cymbidium hybridum as experimental materials, the optimal conditions of Cymbidium hybridum axillary buds regeneration were researched. [ Result]The results indicated that the optimum medium of Cymbidium hybridum axillary buds regeneration was B5 ＋ 6-BA 3.0-4.0 mg/L ＋ KT 0.5 mg/L ＋ NAA 0.1 mg/L ＋ AD 2.0 mg/L ＋ YE 100.0 mg/L. Radication medium adding 2.0 g/L AC and 0.3 mg/L NAA would get well effect, radication rate was 90% and root number reached 3 - 4 items ; whether adding 6-BA had not effect on radieation rate. [ Conclusion] This experiment used axillary buds regeneration model, which have higher genetic stability.