拟南芥花粉体外和体内2种萌发差异的比较研究

Comparison of the Two Germination Differences of Arabidopsis Pollen between in vitro and in vivo

[目的]比较拟南芥花粉体外和体内2种萌发的差异。[方法]以模式植物拟南芥作为研究对象,采用荧光显微镜观察比较2种体外萌发培养基的萌发率差异并比较体外萌发率和体内萌发率的差异。[结果]花粉在固体培养基上的萌发率平均为51.01%,而在液体培养基上萌发率平均为83.97%;在体外,液体萌发培养基优于固体萌发培养基,液体培养基萌发率较固体培养基萌发率高,稳定;体内萌发较体外萌发稳定,萌发率高,平均萌发率为94.00%。[结论]研究结果为检测花粉活力提供了一个有效的检测标准,为拟南芥花粉的生物学研究提供理论与方法。

[ Objective ] Differences of Arabidopsis pollen germination between in vitro and in vivo were compared. [ Method ] The model plant Arabidopsis thaliana was used as study material, and two kinds of culture media for pollen germination in vitro were compared. Also the differences of pollen germination in vitro and in vivo were observed by fluorescence microscope. [ Result] The average germination rate in solid culture was 51.01% and 83.97% in liquid culture, which showed that the germination rate was higher and steadier in liquid culture than that in solid when the Arabidopsis pollens germinated in vitro. Through comparing the differences of Arabidopsis pollens germination rates between in vivo and in vitro, it was found out that the average germination rate in vivo was 94.00% , which was higher and steadier than thatin vitro. [ Conclusion] This research results supplied an efficient detection standard and provided the theory and method for the biology research on Arabidopsis pollen.