摘要
Effects of hypoxia on the dopamme concentration and the immune response of White Shrimp Litopenaeus vannamei were studied. The results showed that hypoxia had significant effects on the concentration of dopamme （DA） in the haemolymph, haemocyte count, phenoloxidase activity, phagocytic activity of haemocytes and bacteriolytic and antibacterial activity in the haemolymph （P〈0.05）. The concentration of the dopamme in haemolymph reached its maximum in the 3.0 and 1.5 mgL^-1 DO groups at 12hand 6h, and then returned to normal after 24h and 12h, respectively. All munune parameters decreased with the reduction of dissolved oxygen. Total haemocyte count （THC）, the hyaline cells and semi-granular cells in the 3.0mgL^-1 DO group became stable after 12h, while granular cells did so after 24h. The THC and different haemocyte count （DHC） in the 1.5mgL^6-1 DO group became stable after 24 h. Phenoloxidase activity and bacteriolytic activity in the 3.0 and 1.5 mgL^-1 DO groups reached their stable levels after 24 h and 12h respectively, while phagocytic activity and antibacterial activity＂ became stable after 24 and 12, and 36 and 24h, respectively. It was also indicated that the changes of dopamme concentrations in haemolymph, haemocyte count and phenoloxidase activity were obviously related to the exposure ttrne under hypoxic conditions.
Effects of hypoxia on the dopamine concentration and the immune response of White Shrimp Litopenaeus vannamei were studied. The results showed that hypoxia had significant effects on the concentration of dopamine (DA) in the haemolymph, haemocyte count, phenoloxidase activity, phagocytic activity of haemocytes and bacteriolytic and antibacterial activity in the haemolymph (P<0.05). The concentration of the dopamine in haemolymph reached its maximum in the 3.0 and 1.5 mg L-1 DO groups at 12 h and 6 h, and then returned to normal after 24 h and 12 h, respectively. All immune parameters decreased with the reduction of dissolved oxygen. Total haemocyte count (THC), the hyaline cells and semi-granular cells in the 3.0 mg L-1 DO group became stable after 12 h, while granular cells did so after 24 h. The THC and different haemocyte count (DHC) in the 1.5 mg L-1 DO group became stable after 24 h. Phenoloxidase activity and bacteriolytic activity in the 3.0 and 1.5 mg L-1 DO groups reached their stable levels after 24 h and 12 h respectively, while phagocytic activity and antibacterial activity became stable after 24 and 12, and 36 and 24 h, respectively. It was also indicated that the changes of dopamine concentrations in haemolymph, haemocyte count and phenoloxidase activity were obviously related to the exposure time under hypoxic conditions.
基金
supported by the Program for New Century Excellent Talents in University(NCET-06-0597)
the Transformation and Expansion of Achievement of Agricultural Science and Technology in Tianjin, China (0604020).
