印度人参根提取物体内抗氧化作用研究

Study on in vivo Anti-oxidation Effects of Indian Ginseng Root Extracts

采用序贯法和霍恩法结合确定提取物LD50并确定实验剂量,选用50只昆明种小鼠随机分成空白对照组、模型对照组和3个剂量组(5.00,2.50,0.80 g/kg.BW),分别灌喂不同剂量印度人参根提取物,30 d后采血测定小鼠和肝损伤小鼠血清丙二醛含量和抗氧化酶活性.结果表明,剂量组小鼠血清MDA含量显著低于空白对照组和模型对照组;血清SOD和GSH-Px活性显著高于空白对照组;对肝损伤小鼠,剂量组MDA含量明显低于模型对照组,而SOD和GSH-Px活性则明显高于模型对照组.表明印度人参根乙醇提取物有一定抗氧化作用,对化学性肝损伤小鼠有一定保护作用.

The Sequential method was used in combination with Horn＇s method to determine LD50 of Indian ginseng extract. Fifty Kunming mice were randomly divided into 5 groups： a blank control group, a model control group and three drug groups（5.00, 2.50, 0. 80 g/kg ·BW）, and administered with Indian ginseng root extract of different concentrations. Blood sam0-les were taken 30 days later to determine malondialdehyde （MDA） content and activity of anti-oxidation enzyme in mice and liver-injured mice. Theresuits showed that MDA content in the drug groups was significantly lower than that in the blank control group and the model control group, while the activities of SOD and GSH-Px in the drug groups were notably higher than those in the blank control group. For the liver-injured mice, MDA content in the drug groups was significantly lower than in the model control group, while the activities of SOD and GSH-Px in the drug groups were notably higher than in the model control group. These results indicated that the extract of Indian ginseng root has the capacity of anti-oxidation effects and has certain protective effect for liver-injured mice.