普罗布考对兔颈动脉血管成形术后血管内膜生长因子的影响及其对再狭窄的干预作用

Effect of Probucol on Restenosis and Growth Factors After Balloon Angioplasty in Rabbits Carotid Artery

目的研究普罗布考对兔颈动脉血管成形术后血管内膜生长因子表达的影响及其对再狭窄的干预作用。方法将32只普通级大耳白兔随机分为高胆固醇组和普罗布考组。喂养1周后以球囊损伤颈总动脉,分别用高胆固醇食物及普罗布考粉剂饲养8周后处死,切取颈总动脉,HE染色观察血管管腔面积和内膜面积的变化,用免疫组织化学法检测胰岛素样生长因子1受体和血管内皮生长因子的表达。结果与高胆固醇组相比,普罗布考组管腔面积明显扩大(P<0.01),内膜面积明显减少(P<0.05),胰岛素样生长因子1受体和血管内皮生长因子表达明显减少(P<0.01)。结论普罗布考显著减轻兔颈动脉血管成形术后再狭窄的形成,抑制胰岛素样生长因子1受体和血管内皮生长因子的表达。

Aim To investigate the effect of pobucol on the prevention of restenosis after balloon angioplasty in hypercholesterolaemic rabbits, and to examine the expression of growth factor. Methods 32 white rabbits were randomly divided into high cholesterol diet group （ n = 16 ） and probucol group （ n = 16 ）, after one week of diet, all rabbits were injured on carotid artery, by balloon angioplasty. Carotid artery tissues were harvested at 8 weeks after injury by balloon. During 8 weeks the rabbits were fed with hypercholesterolaemic diet and probucol. The areas of lumen of blood vessel, intima and elastic fibers dying were observed by HE staining, and the insulin-like growth factor-1 receptor （IGF- 1R）and vascular endothelial growth factor （VEGF） were examined by immunohistochemical method. Results Compared with the high cholesterol diet group, the lumen areas of the probucol group were larger （P 〈 0.01 ）, the intimal areas were smaller （ P 〈 0.05 ）, and the expression of IGF-1R and VEGF significantly decreased （ P 〈 0.05 ）. Conclusions Probucol can prevent the restenosis of rabbits＇ carotid artery from balloon angioplasty injury, and inhibit the expression of IGF-1R and VEGF.