转化生长因子β1及其2型受体在实验性大鼠升主动脉瘤中的表达

EXPRESSIONS OF TRANSFORMING GROWTH FACTOR-BETA 1 AND ITS TYPE Ⅱ RECEPTOR IN EXPERIMENTAL RAT ASCENDING AORTIC ANEURYSM

目的研究转化生长因子β1(TGFβ1)及其2型受体(TGFβRⅡ)在实验性大鼠升主动脉瘤中的表达及其意义。方法缩窄幼年Wistar大鼠升主动脉制作升主动脉瘤模型,4个月后处死动物,用免疫组织化学和Western blotting方法检测动脉瘤壁TGFβ1和TGFβRⅡ的表达。结果免疫组织化学显示,TGFβ1表达于动脉瘤和对照组动脉全层;TGFβRⅡ大量表达于动脉瘤增生的内膜和中膜平滑肌层,而对照组表达很弱。Western blotting显示,动脉瘤组织中TGFβ1和TGFβRⅡ表达均强于对照组。结论TGFβ1和TGFβRⅡ在动脉瘤中表达强于对照组,TGFβ信号通路可能在动脉瘤形成过程中起重要作用。

Objective To investigate the expressions and significance of transforming growth factor-beta 1 （TGFβ1） and its typeⅡ receptor （TGFβRⅡ） in experimental rat ascending aortic aneurysm of rat model. Methods The rat ascending aortic aneurysm models were made by banding ascending aorta of young Wistar rats. The ascending aortas were taken 4 months after operation, lmmunohistochemistry staining and Western blotting were used to investigate the expressions of TGFβ1 and TGFβR Ⅱ . Result Immunohistochemistry staining results showed that TGFβI expressed in all layers of the aortic aneurysm and the control. TGFβR Ⅱ was extensively located in the hyperplastic intima and tunica media smooth muscle cells in the aortic aneurysm, while there was only a little positive staining in the control group. Western blotting results indicated that the expression levels of TGFβ1 and TGFβR Ⅱ in the aortic aneurysm were stronger than the control, P 〈 0.05 and P 〈 0.01 respectively. Conclusion The expressions of TGFβ1 and TGFβR Ⅱ in ascending aortic aneurysm are stronger than that in the control. The TGFβ signaling may contribute to the development of aortic aneurysm.