小鼠精子细胞变态成形过程中manchette的免疫荧光染色分析

IMMUNOFLUORESCENCE ANALYSIS OF MANCHETTE DURING MOUSE SPERMIOGENESIS

目的通过观察manchette结构在小鼠精子细胞中的定位和形态变化,探讨其在小鼠精子细胞变态成形过程中的作用和意义。方法利用免疫荧光FITC/DAPI共染技术显示manchette结构在小鼠精子细胞变态成形各阶段的细胞内定位,观察精子细胞成熟过程中manchette结构的形态学改变。结果manchette结构紧密附着在精子细胞核表面;该结构在圆形精子细胞核变形和延伸的起始阶段形成,并随着精子细胞核的浓缩和变长逐渐向精子细胞尾部移位,直至精子变态成形后消失;在精子细胞变态成形过程中,manchette随着精子细胞核形态的改变逐渐从"帽状"结构变形为"微管样"结构。结论Manchette结构的形成和消失与精子细胞核的浓缩及延伸同步,其形态变化和位置改变与精子细胞核的形态学变化相吻合,在小鼠精子细胞变态成形过程中具有重要意义。

Objective To investigate spermiogenesis. Methods the localization and the morphological changes of manchette during mouse staining with FITC and costaining with DAPI were used to demonstrate the cellular localization of the manchette at different stages during mouse spermiogenesis. The structural changes of the manchette were observed during the maturing of the spermatid. Results staining showed that manchette existed exactly around the nuclei of the spermatids. Manchette began to form, when the shape of the nucleus changed from spherical to slightly elongated. While the nucleus of the spermatids condensed and elongated at later stages, manchette moved gradually to the caudal position of the spermatids. At last, the manchette diminished as the spermatids became mature. During mouse spermiogenesis, manchette underwent a transition from a cap-like to a tubular configuration. CondusionThe formation and diminishment of the manchette is in step with the condensation and elongation of the nucleus of the spermatid. Both the structural and positional changes of the manchette coincide with the changes of the nucleus. These results imply that manchette might play an important role in mouse spermlogenesis