叉头框C2在人胚淋巴管发生过程中的表达

EXPRESSION OF FOXC2 DURING LYMPHANGIOGENESIS IN HUMAN EMBRYO

目的探讨人淋巴管胚胎期的发生和发育及叉头框C2(FOXC2)的表达。方法用淋巴管内皮透明质酸受体1(LYVE-1)作为淋巴管的标记物,应用免疫组织化学SP染色及免疫荧光双标染色的方法,检测FOXC2和LYVE-1在妊娠5～11周的85例人胚胎标本淋巴管的表达及淋巴管的发生发育情况。结果妊娠7周后的胎儿颈部及胸部的淋巴管出现LYVE-1表达。在妊娠大约10周时,胎儿肠系膜间淋巴管内皮细胞开始出现LYVE-1表达。FOXC2的表达早于LYVE-1,于妊娠第6周开始在中胚层间充质明显表达。FOXC2不仅表达于淋巴管,并广泛分布于椎体、心血管等部位。妊娠11周后的胎儿淋巴管内皮细胞中仍能见到FOXC2和LYVE-1的表达。结论在妊娠7～8周间(人胚胎发育35～42d),淋巴管开始形成,FOXC2和LYVE-1与淋巴管的发生和发育相关。

Objective To study the human lymphangiogenesis in the early stage of embryo and the expression of forkhead box C2（FOXC2） in human lymphangiogenesis. Methods Lymphatic vessel endothelial haluronic acid receptor-1 （LYVE-1） was used as the special marker of lymphatic vessels. Immunohistochemical and double immunofluorescence staining were used to detect the expressions of FOXC2 and LYVE-1 in lymphatic vessels of 85 human embryos of 5 to 11 weeks pregnancy and analyze the features of lymphatic vessel genesis and development. Results LYVE-I was initially detected in lymphatic vessels in 7-week human embryos. The lymphatic vessel endothelial cells presented brown staining for LYVE-1 in jugular and thoracic region. LYVE-1 was also found to express in human embryonic mesentery lymphatic vessels on the 70th day of pregnancy. The expression of FOXC2 in human embryo was detected prior to that of LYVE-1. At the beginning of the 6th week of pregnancy, FOXC2 was obviously seen in human embryonic mesoderm mesenchyme. FOXC2 expressed not only in human embryonic lymphatic vessels, but also in the vertebral body, cardiovascular wall and other tissues. The expressions of FOXC2 and LYVE-1 were still seen in human embryonic lymphatic vessel endothelial cells after 80 days of pregnancy. Conclusion Lymphangiogenesis of human embryo begins between the 7th anti 8th weeks of pregnancy. FOXC2 anti LYVE-1 could have some relation with the human embryonic lymphatic vessel genesis and development.