摘要
目的通过确定卵泡刺激素(FSH)与神经生长因子(NGF)或表皮生长因子(EGF)在大鼠下颌下腺的共定位关系,研究FSH在体外对大鼠下颌下腺组织分泌NGF和EGF的影响。方法采用邻片免疫组织化学共定位方法;体外孵育大鼠下颌下腺组织并给予不同浓度FSH,应用酶联免疫分析方法检测上清液中NGF和EGF的含量。结果大鼠下颌下腺浆液性腺上皮细胞、颗粒曲管及大于颗粒曲管的导管上皮细胞均呈FSH、NGF及EGF免疫反应阳性,FSH与NGF或EGF有共存性;当FSH浓度大于10-5～10-6时,NGF或EGF分泌量随FSH浓度的递减而降低;当FSH浓度小于10-5～10-6时,NGF或EGF分泌量随FSH浓度的递减而升高。结论FSH在体外对NGF或EGF分泌具有相同的双向调节作用,FSH可能对下颌下腺内分泌具有调节功能。
Objective To investigate the distribution of nerve growth faetor（NGF） and epidermal growth factor （EGF） and their colocalization with follicle-stimulating hormone （FSH） in rat submaxilary glands, and to study the effects of FSH on the secretion of NGF and EGF by submandibular gland tissue in vitro. Methods Immunohistochemical colocalization method was used in the experiment. Submandibular tissues of rats were incubated in vitro and different concentration of FSH was added. Enzyme link immunoassay was used to measure EGF and NGF in supernatant. Results The serous glandular cells, granular convoluted epithelial cells and all other duct epithelial cells in the submandibular gland showed FSH, NGF or EGF positive The positive substance was distributed in the cytoplasm with negative nuclei. Both FSH and NGF or EGF positive products were colocated in rat submandibular gland. When the concentration of FSH was more than 10^-5-10^-6 IU/L, the secretion of EGF and NGF lessened with the decreased concentration of FSH; when the concentration of FSH was less than 10^-5-10^-6IU/L, the secretion of EGF and NGF increased with the decreased concentration of FSH. Conclusion FSH has the same bidirectional regulation effects on EGF and NGF in vitro. FSH may regulate the function of endocrine of rat submandibular gland.
出处
《解剖学报》
CAS
CSCD
北大核心
2008年第6期927-930,共4页
Acta Anatomica Sinica
