对微球囊栓塞大脑中动脉制作猕猴局灶性脑缺血再灌注模型的评价

THE EVALUATION OF FOCAL CEREBRAL ISCHEMICA REPERFUSION MODEL WITH MIDDLE CEREBRAL ARTERY OCCLUSION BY MICRO-BALLOON IN RHESUS MONKEYS

目的建立一种理想的猕猴局灶性脑缺血再灌注模型。方法成年健康猕猴12只(雌雄各半)。经颈总动脉或股动脉介入手术,将标准微球囊导管插入大脑中动脉(MCA)的起始部,然后充盈微球囊阻断MCA血流,退出微球囊后实现MCA血流再灌注,建立大脑中动脉闭塞再灌注(MCAO/R)模型。通过脑血管造影、磁共振血管成像(MRA)、磁共振扫描成像(MRI)、氯化三苯基四氮唑(TTC)染色和神经行为功能评分对动物模型进行评价。结果经颈总动脉或股动脉介入手术,可以在荧光屏直视下准确地将微球囊导管插入大MCA阻断其血流,MCA在MRA上不显像。MCA供血区磁共振T1、T2、DWI出现高信号区,TTC染色显示脑梗死病灶,动物出现相应的神经功能障碍。该方法成功率高、重复性好、操作简单。结论经股动脉微球囊导管介入手术建立猕猴MCAO/R模型是一种较为理想的方法。

Objective To establish an ideal focal cerebral ischemia reperfusion model in monkeys. Methods Adult healthy rhesus monkeys （Macaca mulatta ） 12 cases （male 6 and female 6）, aged 4-7 years and weighted 4.8-7.5kg. were used in this study. The middle cerebral artery occlusion/reperfusion （MCAO/R） model was established by inserting a standard micro-balloon catheter intraluminally from the carotid common artery or femoral artery into the proximal segment of the middle cerebral artery （MCA）. The regional cerebral blood flow of MCA was occluded by expanding the micro-balloon to cause ischemia, and withdrawing the micro-ballon catheter to reperfuse the MCA. The MCAO/R model was evaluated by angiography, magnetic resonance angiography （MRA）, magnetic resonance imaging （MRI）, tetrazolium chloride （Trc） staining and neurological behavoral function scores. Results By inserting a micro-balloon catheter intraluminally from the carotid common artery or femoral artery into the MCA, the micro-balloon catheter could be inserted into the MCA to occlude blood flow, and no image of MCA shown on TV screen. In MCA blood flow supplied area, magnetic resonance T1, T2 and DWI showed high signals, 3TC staining showed cerebral ischemic infarction, and correspondly the monkeys showed neurological function disorders. This method used a simple operatirc procedure had a high successful rate, and could be repeated. Conclusion We showed ideal method to establish the MCAO/R model in monkeys by inserting intraluminally a micro-balloon catheter into the MCA.