血管内皮生长因子936^＊T／C基因多态性与原发性肝癌的关系

Relation of VEGF gene 936^＊ T/C polymorphism to primary hepatocellular carcinoma

目的通过研究血管内皮生长因子（VEGF）936^＊T／C基因多态性与原发性肝癌之间的关系，了解该基因多态性对原发性肝癌生成及发展的影响。方法采用聚合酶链反应限制性片段长度多态性（PCR—RFLP）方法检测原发性肝癌及正常对照的VEGF936^＊T／C基因型。结果原发性肝癌病人VEGF936^＊T／C基因型或等位基因与对照组相比无差异（精确概率法计算基因型P=0．275；卡方检验等位基因χ^2=0．877，P=0．349）。在肿瘤直径≥8cm的病人C／C基因型和C等位基因比例（66．7％和82．O％）明显大于肿瘤直径〈8cm病人的比例（40．0％和65．0％），两者差异有统计学意义（C／C基因型χ^2=4．722，P=0．029；C等位基因χ^2=5．393，P=0．020）。结论VEGF936^＊C／C基因型或936^＊C等位基因与原发性肝癌的生成无关，但VEGF936^＊ C／C基因型和C等位基因有助促进肿瘤的生长。

Objective To investigate the relation of VEGF gene 936^＊ T/C polymorphism to pri- mary hepatocellular carcinoma. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was employed to study the VEGF 936^＊ T/C genotypes in patients with primary hepatocellular carcinoma and healthy controls. Results There was no significant difference in the frequency of VEGF 936^＊ C/C genotype or C allele between the patients and healthy controls （genotype P=0. 275; allele χ^2 =0. 877,P=0. 349）. The C/C genotype or C allele in patients with tumor diameter≥8 cm （66.7% and 82.0% ） were more frequently found than in those with tumor diameter〈 8 cm （40.0% and 65.0%）, there was significant difference （C/C genotype χ^2 =4. 722,P=0. 029;C allele χ^2=5. 393,P=0. 020）. Conclusion VEGF 936^＊ C/C genotype or C allele is not related to the development of primary hepatocellular carcinoma, but they both are related to the growth of the carci-