摘要
目的观察CYP2J3基因转染后大鼠心、肝、肺、肾及胸主动脉CYP2J3基因表达和11,12-EET的含量。方法经大鼠阴茎背静脉快速注射质粒复制大鼠转基因模型。将36只雄性Wistar大鼠(280～300g)随机分为空白对照组、pcDNA3.1质粒和pcDNA3.1-CYP2J3重组质粒注射组,分别于14和28d取材。用半定量RT-PCR法检测CYP2J3mRNA表达,高效液相色谱法测定11,12-EET含量。结果pcDNA3.1-CYP2J3重组质粒组28d时各组织CYP2J3基因表达强于对照组和pcDNA3.1质粒组;各组织11,12-EET含量增高(P<0.05)。结论以pcDNA3.1质粒作为非病毒性载体,可增强目的基因CYP2J3在心、肺、肝、肾及胸主动脉的表达。
Objective To detect CYP2J3 gene expression and contents of 11,12-EET in heart, liver, lung, kidney and aorta thoracalis after CYP2J3 gene transfection. Methods The rat transgenic model was developed by injecting plasmid through vena dorsalis penis. The animals were divided into control group, pcDNA3.1 transgenic gToup and pcDNA3.1-CYP2J3 transgenic group. The expression of CYP2J3 mRNA was detected by RT-PCR and content of 11,12-EET was examined by the HPLC at 14 days and 28 days after injection. Results Twenty eight days after injection, both expression of CYP2J3 mRNA and the content of 11,12-EET were significantly increased as compared with that of control and pcDNA3.1 transgenic group （P 〈 0. 05）. Conclusion Plasmid injection can increase the expression of CYP2J3 in heart, liver, lung, kidney and aorta thoracalis of rats.
出处
《基础医学与临床》
CSCD
北大核心
2008年第12期1251-1254,共4页
Basic and Clinical Medicine
基金
北京自然科学基金(7062007)
