不同胚龄北京鸭视网膜节细胞层细胞大小、数量及密度

Cell size,number and density in the retina ganglion cell layer of Pekin duck retina at different embryonic age

采用视网膜铺片Nissl染色法和Scion Image图像分析法,研究北京鸭胚胎期11(E11)、14、17、20、23和26日龄视网膜节细胞层(Ganglion cell layer,GCL)细胞大小、数量及密度分布变化。结果表明:E11-E14,GCL细胞小,均呈圆形,E17开始出现大细胞;E11至E26视网膜中央区(CA)细胞大小增长1.97倍,而颞侧周边区(TP)增长3.1倍,相邻胚龄间细胞大小差异显著。GCL细胞总数在E17达到最大值2.03×106个,随后细胞数量显著下降。视网膜中央-周边密度梯度在E11出现。CA细胞密度在E17达到最大值2.54×104个/mm2,随后密度下降;周边区细胞密度随胚龄增长而持续下降,且TP细胞密度下降最显著。结果提示,北京鸭胚胎发育过程中,GCL细胞大小、数量及密度均发生显著变化,而E17是其中的一个重要转折点。

Changes in cell size, number and density of the ganglion cell layer （GCL） of the Pekin duck retina were studied by using the methods of Nissl-staining and Scion Image picture processing at embryonic age day-11, 14, 17, 20, 23 and 26. The result indicated that the cells in GCL were small and round uniformly at Ell and E14. The large cells were first found at E17. From E11 to E26, cell size was increased 1.97-fold in the central area （CA） and 3.1-fold in the temple periphery （TIP）, and there was significant difference among all embryonic ages. During the growth and development of embryo, the total number of cells in GCL was increased to 2.03 × 10^6 cells at E17, and then decreased significantly with age. Differentiation of the centralperipheral gradient in cell density has been performed at Ell. Cell density in CA was up to the maximal value （2.54 × 10^4 cells/ mm^2 ） at E17 and then decreased. However, cell density was decreased constantly in the peripheral area, especially in TP. In conclusion, E17 is the most important point for the retina during the embryonic developmental of Pekin duck, accompanying changes in cell size, number and density in GCL [ Acta Zoologica Sinica 54 （6）： 1082- 1088, 2008 ].