Bim基因在大肠癌中的表达

Expression of Bim gene in colorectal cancer

目的:探讨大肠癌组织中Bim基因的表达及其与大肠癌生物学特征和预后的关系.方法:收集哈尔滨医科大学附属肿瘤医院2001-2002年手术切除且资料完整的大肠癌组织标本98例,均经根治性手术治疗和病理确诊.同时取癌组织及癌旁5cm处正常肠组织.采用免疫组化SP法检测Bim蛋白大肠癌及正常大肠组织中的表达.结果:Bim的阳性表达率在大肠癌组织中明显低于癌旁组织,差异有统计学意义(28.57%vs98.97%,P<0.05).Bim蛋白表达下降或完全丧失与大肠癌的浸润深度、淋巴结转移及临床分期有关(χ2=6.959,35.381,4.905,均P<0.05),而与患者的年龄、肿瘤大小、组织学类型、分化程度无关.Bim表达阳性患者的生存率高于表达阴性患者的生存率(P<0.05).Bim阳性表达者,术后平均生存时间比Bim表达阴性者长1.03mo.结论:Bim在大肠癌的发生发展中起重要作用,对评价患者的预后有一定价值.

AIM： To study the expression of Bim gene in human colorectal cancer and to examine the association between the Bim expression, and the clinicopathological parameters and prognosis. METHODS： Ninety-eight colorectal cancer cases with complete data were collected at the affiliated tumor hospital of Harbin Medical University from 2001 to 2002. All cases had received radical surgery and were pathologically confirmed. At the same time, cancer tissue and normal tissue 5 cm away were analyzed for Bim protein expression using immunohistochemical SP method. RESULTS： The positive rate of Bim protein expression was significantly lower in colorectal cancer than in controls （28.57% vs 98.97%, P 〈 0.05）. The expression of Bim protein was correlated to the invasive depth, lymph node metastasis and clinical staging （;2 = 6.959, 35.381, 4.905, all P 〈 0.05）, but not to the age, tumor size, tumor types or tumor differentiation. The survival rate of the patients with positive Bim protein was higher than with negative Bim protein （P 〈 0.05）. Survival time of the patients with positive Bim protein after operation was I mo longer than negative Bim protein. CONCLUSION： Bim may play an important role in the carcinogenesis and progression of colorectal carcinoma. The expression of Bim protein has a definite value in evaluating the prognosis.