海洋细菌增菌培养方法的研究

Cultural methods of enrichment in ocean bacteria

目的探讨理想的海洋细菌增菌培养方法。方法选择不同种类的增菌培养液对海水中细菌进行增菌培养。结果碱性蛋白胨水与嗜盐菌增菌液对海水弧菌具有良好的增菌效果，2号营养肉汤适合海水普通细菌的增菌培养，液体沙保氏增菌液能从海水中增菌培养出真菌。结论海水中弧菌增菌应选用碱性蛋白胨水与嗜盐菌增菌液，增菌普通细菌应选用2号营养肉汤，真菌应选用液体沙保氏增菌液。

Objective To explore the effecitve cultural methods of enrichment in ocean bacteria. Methods Different kinds of cultural mediums were selectd to culture seawater bacteria. Results Both alkaline peptone water（APW） and halophilic bacteria liquid had good effects to Vibrio enrichment. NO. 2 nutrient broth was effective for ordinary bacteria. Eumycete could be isolated from seawater by cultural sabouraud liquid. Conclusions APW and halophilic bacteria liquid should be selected for Vibrio enrichment, NO. 2 nutrient broth for ordinary bacteria. Moreover, cultural sabouraud liquid can be effective for fungus.