摘要
以高感稻瘟病的低海拔粳稻江南香糯与高抗稻瘟病的高原粳稻子预44作亲本进行杂交,获得F1、F2、BC1F1和以F2单粒传构建的、由276个株系组成的F7重组自交系群体。分别用稻瘟病菌生理小种ZB13和ZE1对两亲本江南香糯和子预44及其杂交后代群体F1、F2和BC1F1进行抗性接种鉴定和遗传分析。结果表明,子预44对稻瘟病菌生理小种ZB13和ZE1的抗性都表现为单基因控制的显性遗传。进一步利用重组自交系F7群体的266个有效株系作为定位群体,将抗稻瘟病菌生理小种ZE1的基因定位在水稻第11染色体上与SSR标记RM206间遗传距离为0cM的位置,暂定名为Pi-zy(t)。这些结果为进一步将子预44中的抗性基因用于水稻抗病育种及该抗稻瘟病基因的克隆奠定了重要基础。
The F1 , F2 , BC1 F1 and 276 F7 recombinant inbred lines (RILs) derived from Ziyu 44(a plateau japonica rice variety [rom Yunnan Province, China, with durable blast resistance) and Jiangnanxiangnuo (a low latitude japonica rice variety, highly susceptible to blast) were obtained to study the inheritance of rice blast resistance. The F1 , F2 and BC1 F1 were inoculated with blast races ZB13 and ZE1, respectively. Genetic analyses on the three populations of Fl, F2 and BC1 F; showed that the rice blast resistance to the races ZB13 and ZE; were both controlled by a single dominant gene, respectively. Furthermore, a population including 266 F7 RILs from the 276 F7 RILs was used to map the rice blast resistance gene to the race ZE1. The gene was mapped on rice chromosome 11 with a distance of 0 cM to SSR marker RM206 and tentatively designated as Pi-zy (t). These results provide essential information for further utilization of the blast resistance gene in Ziyu 44 in rice disease resistance breeding and positional cloning of the gene.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2009年第1期31-35,共5页
Chinese Journal of Rice Science
基金
云南省中青年学术技术带头人培养计划资助项目(2004PY01-12)
云南省教育厅自然科学基金重点资助项目(2006ZB007)
国家科技支撑计划资助项目(2006BAD01A01-1)
关键词
粳稻
稻瘟病
抗性基因
重组自交系
基因定位
japonica rice
rice blast
resistance gene
recombinant inbred line
gene mapping