人工种植黄芩与野生黄芩叶中Se含量及氨基酸含量的比较研究

Comparative Study on Selenium and Amino Acids Content in Leaves of Planted and Wild Scutellaria Baicalensis

采用荧光光度法测定了人工种植黄芩和野生黄芩叶中微量元素Se的含量,氨基酸分析仪测定了18种氨基酸的含量,结果表明人工种植黄芩叶与野生黄芩叶中皆含有较高的Se,但其含量没有显著差异(α=0.05)。黄芩叶中富含氨基酸,其中含量较多的是天冬氨酸、谷氨酸、亮氨酸,人工种植黄芩叶氨基酸含量高于野生黄芩叶,说明人工种植黄芩可以替代野生黄芩用于黄芩叶产品的开发。实验结果为揭示黄芩的生物功能以及比较人工种植黄芩和野生黄芩叶中Se及氨基酸的差异提供有用数据,并为开发黄芩叶食用、药用价值提供理论依据。

Scutellaria baicalensis is one of the most important Chinese herbs.It is widely used in Asian medicine to improve impaired brain function and to treat headaches,and used to treat prostate cancer.It is also known to be anti-inflammatory and antifungal,and also seems to have antiviral properties,including possible effectiveness against HIV.Scutellaria baicalensis tea and other products are in development.In the present study,the content of selenium（Se） in leaves of planted and wild Scutellaria baicalensis was determined by fluorescence photometer.The contents of 18 kinds of amino acids in the leaves of planted and wild Scutellaria baicalensis were determined with amino acids instruments.The results showed that the two kinds of leaves were rich in Se content,and the content of Se in planted Scutellaria baicalensis（0.051 μg·g^-1） was not significantly different from that in wild one（0.051 μg·g^-1,α=0.05）.The amino acids,of which the total content was up to 14.62% and 10.25% separately,were rich in both planted and wild Scutellaria baicalensis.Among the 18 kinds of amino acids,aspartic acid,glutamic acid and leucine were comparatively high in leaves of planted and wild Scutellaria baicalensis.There are 8 kinds of amino acids essential to human body,which were higher in leaves of planted Scutellaria baicalensis than those of wild one.This study,for the first time,determined Se and amino acids content in Scutellaria baicalensis and concluded that the leaves of planted type have Se and amino acids content not lower or higher than that of wild type,and the planted type could be a good substitute of wild type in the development of Scutellaria baicalensis products.This study also provided useful data for explaining the multifunction of Scutellaria baicalensis and theological basis for developing its medical and edible value.