单侧颈内动脉注入 MPTP 建立偏侧帕金森病灵长类模型的研究

The Study of Hemiparkinsonism in Primate after Unilateral Internal Carotid Artery Infusion of MPTP

分别向未夹闭颈外动脉（Ａ组）和夹闭颈外动脉（Ｂ组）的恒河猴（Ｍａｃａｑｕｅ）的一侧颈总动脉注入甲基苯基四氢吡啶（ＭＰＴＰ），制造帕金森病（ＰＤ）灵长类动物模型。发现未夹闭组动物均需重复注射ＭＰＴＰ２～４次方可出现ＰＤ症状；而夹闭组动物均经１次手术获得成功。术后１个月脑核磁共振检查显示注射侧黑质区域密度减低、面积缩小。抗酪氨酸羟化酶免疫组化脑切片显示手术侧中脑多巴胺能神经元明显少于对侧。研究结果表明：夹闭颈外动脉向颈总动脉注入ＭＰＴＰ，是一种简便有效的建立灵长类偏侧帕金森病模型的方法。

n order to make a primate model for Parkinsonizm,13 monkeys (Macaque) were ipsilaterally injected through the common carotid artery by 1methyl4phenyl1,2,3,6tetrahydropyridine(MPTP). Among them, the extrenal carotid artery in 6 monkeys was clipped before the injection. All animals with symptoms showed to have a decrease of density in the area of the substantia nigra on the image of MRI after 1 month of injection. In addition, the tyrosine hydroxylaseimmunoreactive neurons in the ventral midbrain area on the injection side were decreased in these monkeys. The results showed that ipsilateral injection of MPTP into the common carotid artery with a temporary blockage of the external carotid artery was a good technique for producing the primate model for Parkinsonizm.