糖尿病视网膜病变与血管紧张素转换酶基因多态性的关系

Association between ACE Gene Polymorphism and Diabetic Retinopathy

为探讨血管紧张素Ⅰ转换酶（ＡＣＥ）基因插入／缺失（Ｉ／Ｄ）多态性与非胰岛素依赖型糖尿病（ＮＩＤＤＭ）及其视网膜病变的关系，以ＡＣＥ基因第１６内含子１个２８７ｂｐＡｌｕ顺序Ｉ／Ｄ型为多态性标志，用聚合酶链反应（ＰＣＲ）扩增基因片段，１％琼脂糖凝胶电泳ＰＣＲ产物。结果８７例ＮＩＤＤＭ与５０例正常人之间基因频率无显著差异；ＮＩＤＤＭ合并视网膜病变与未合并视网膜病变２组间基因型频率和等位基因频率无显著差异。提示糖尿病视网膜病变与ＡＣＥ基因多态性无关。

o clarify whether angiotensinconverting enzyme(ACE) insertion(I)/deletion(D) gene polymorphism attributes to the development of noninsulin dependent diabetes mellitus(NIDDM) and diabetic retinopathy. A fragment of 287 bp Alu sequence in inron 16 of ACE gene was used as I/D polymorphic marker. After PCR (polymerase chain reaction) amplication of DNA fragment, 1% agarose gel electrophoresis was undertaken to analyse the PCR products. Results: There was no significant difference in the frequency of ACE genotypes between 87 NIDDM patients and 50 normal subjects. There was no significant difference in the insertion(I) and deletion(D) alleles or frequencies of DD, DI, or II genotypes between two subgroups of NIDDM patients with and without diabetic retinopathy respectively (P<0.02). These results suggested that there was no association between ACE gene polymorphism and diabetic retinopathy.