亮-脑啡肽对大鼠缺血皮层β-内啡肽和精氨酸加压素的影响

Effects of Leu-enkephalin on Arginine Vasopressin and β-endorphin Contents in Cerebral lschemic Cortex of the Rat

本研究应用四血管结扎大鼠全脑缺血模型，借助放射免疫分析法，观察了大鼠脑缺血后皮层亮－脑啡肽（ＬＥＫ）、β－内啡肽（β－ＥＰ）和精氨酸加压素（ＡＶＰ）含量的动态变化，在此基础上进一步观察了预先侧脑室注射亮－脑啡肽抗血清（ＬＥＫ－ＡＳ）对脑缺血后皮层β－ＥＰ、ＡＶＰ含量的影响。结果显示：ＬＥＫ的含量在脑缺血早期明显升高而后迅速降低，β－ＥＰ、ＡＶＰ则随着缺血时间的延长逐渐升高；预先予侧脑室注射ＬＥＫ－ＡＳ后，缺血皮层β－ＥＰ、ＡＶＰ的含量明显降低。研究结果提示：ＬＥＫ、β－ＥＰ、ＡＶＰ均参与脑缺血的病理生理过程，ＬＥＫ可能通过影响ＡＶＰ或／和β－ＥＰ而间接参与脑缺血的病理生理过程。

By radioimminoassay,on the fourvessel occlusion model in the rat,the dynamic changes of Leuenkephalin (LEK)、βendorphin (βEP) and arginine vasopressin (AVP) contents in cortex after cerebral ischemia and the effects of preliminary intraventricular injection of LEK antiserum (LEKAS) on βEP and AVP contents in ischemic cortex were observed. The results showed that,after cerebral ischemia,LEK contents increased at first and then dropped quickly,βEP and AVP contents increased gradually. The results also demonstrated that preliminary intraventricular injection of LEKAS might inhibit the increase of βEP and AVP contents in ischemic cortex. This study suggests that LEK、βEP and AVP all participate in the pathophysiological process of cerebral ischemia,and LEK might participate in the process indirectly by exerting influence on AVP and βEP.