丁基苯酞对正常及局部脑缺血大鼠纹状体脑血流影响

Effect of dl-3-n-butylphthalide on striatum cerebral blood flow in normal and middle cerebral artery occlusion rats

应用氢清除法连续测定大鼠一侧纹状体脑血流量（ｒＣＢＦ），并观察丁基苯酞（ＮＢＰ）对其影响．结果表明，ＮＢＰ４０或８０ｍｇ·ｋｇ－１ｉｇ均能显著增加大脑中动脉阻断后纹状体的ｒＣＢＦ（与溶剂对照组相比Ｐ＜０．０１），作用持续３ｈ．此外，５－２０ｍｇ·ｋｇ－１ｉｇ时，也可增加正常大鼠纹状体脑血流，各剂量组ＮＢＰ对平均动脉压均无明显影响；尼莫地平（Ｎｉｍ）０．５ｍｇ·ｋｇ－１ｉｐ也能明显增加纹状体脑血流，同时轻度降低动脉平均压，其增加脑血流作用与ＮＢＰ５ｍｇ·ｋｇ－１的作用相当．提示ＮＢＰ不仅能增加正常大鼠纹状体的脑血流，还能增加大脑中动脉阻断后纹状体的血流量，其抗脑缺血作用可能与该作用有关．

The purpose of this study was to observe the changes in regional cerebral blood flow(rCBF) after administration of dl-3-n-butylphthalide(NBP) in normal or middle cerebral artery occlusion(MCAO) rats. Microelectrode was implanted in right striatum of chloral hydrateanesthetized rats and rCBF was monitored by hydrogen clearance method. The results indicated that NBP could markedly enhance the regional blood flow to striatum ipisilateral to MCAO. Both NBP 40 and 80 mg·kg1(ig 10 min after MCAO)treated group showed sustained increase in regional blood flow to right striatum(P<0.01). In addition, NBP significantly increased the regional blood flow to striatum in normal rats when 5-20 mg·kg1(ip) was used, and it was reached the maxium effect at the dosage of 10 mg·kg1, but it had a weaker effect at the dosage of 20 mg·kg1. No changes in mean arterial blood pressure(MABP) was found during the experiments. When nimodipine(0.5 mg·kg1) was ip administered, there was a similar improvement of rCBF with a slight decline in MABP. These data suggests that NBP can increase rCBF without alteration in MABP, and its effects on cerebral microvasculature are likely to contribute to its neuroprotective effects.