乙型病毒性肝炎患者血清IFN-Υ和TNF-α的检测及意义

The Detection of Interleukin and Tumor Necrosis Factorα in Serum of Patients with Viral Heratitis B and its Clinical Singnificance

目的：探讨ＩＦＮ－和ＴＮＦ－α在乙型病毒性肝炎发病机制中的作用。方法：采用双抗体夹心ＥＬＩＳＡ法对９６例乙型肝炎患者和３０例健康献血员血清中ＩＦＮ－和ＴＮＦ－α进行检测，用ｔ检验进行统计学分析和相关性分析。结果：各型乙型肝炎患者血清ＩＦＮ－和ＴＮＦ－α水平明显高于对照组（Ｐ＜０．０５）；重型肝炎组（ｘＩＦＮ＝２３４．７±１６．５；ｘＴＮＦ＝３５９．０±１７．２ｐｇ／ｍｌ）与急性肝炎组（ｘＩＦＮ＝１７４．９±１２．０；ｘＴＮＦ＝２２０．６±８．９ｐｇ／ｍｌ）比较，前者均明显高于后者，差异显著（Ｐ＜０．０５）；各型乙型肝炎患者ＩＦＮ－和ＴＮＦ－α呈正相关（ｒ＝０．２４，Ｐ＜０．０５）。结论：ＩＦＮ－和ＴＮＦ－α异常增高可能与肝损伤发生有关。

Objective: Studing the effects of IFN and TNFα in pathogenesis of viral hepatitis B. Methods: IFN and TNFα in sera from 96 patients with viral hepatits B and 30 healthy persons were detected by ELISA. The statistical analysises were made by t test. Results: IFN and TNFα in sera from HBV infectors were significantly higher than those in healthy persons (P<005); IFN and TNFα values in patients with fulminant hepatitis were obviously higher than those patients with acute hepatitis (P<0.050; IFN closely related to TNFα (r=0.24,P<0.05). Conclusion: Enhanced IFN and TNFα in sera of patients with HBV infection was involved in liver damage.