凝集素受体在叶酸、维生素C拮抗环磷酰胺致神经管畸形中的作用研究

THE ROLE OF LECTIN RECEPTORS IN FOLIC ACID AND VITAMIN C WHICH PREVENTS NEURAL TUBE DEFECTS INDUCED BY CYCLOPHOSPHAMIDE IN RATS

目的:为探讨凝集素受体在叶酸,维生素C防止环磷酰胺致大鼠胚胎神经管畸形中的作用.方法:实验组(叶酸、维生素C预防组)和对照组孕鼠均于给环磷酰胺后24小时剖腹取胎.常规石蜡包埋切片.用刀豆凝集素.花生凝集素和荆豆凝集素为分子探针作亲合组织化学染色.对两组胚胎神经管的神经上皮,界膜及神经管周围间充质相应糖基进行定性.定位和半定量的比较观察.结果:对照组胚胎的刀豆凝集素和花生凝集素染色明显增强.说明环磷酰胺可使神经上皮细胞表面相应的受体糖蛋白增高.从而干扰了神经上皮的正常细胞增殖、迁移、粘着和识别过程.导致神经管畸形.结论:实验组的染色明显弱于对照组,说明叶酸,维生素C可抑制相应的受体糖蛋白的异常增高,从而使神经上皮的正常细胞增殖、迁移等过程不受明显影响,防止神经管畸形的发生.

In order to explore the roles of lectin receptors in folic acid and vitamin C which prevents neural tube defects(NTD) induced by cyclophosphamide(CP). pregnant rets in the treated group (folic acid and vitramin C) and the control group were dissected at 24 hours after being given CP. The embryos were routinely embedded with paraffin and sectioned in series. The ConA. PNA. and UEA were used as molecule probes to explore the related oligosaccharides-lectin receptors of the neural epithelium, the limiting membrane and the mesenchyme around the neural tube and a comparative observation was made on their quality, distribution and semiquantity. The results showed great stain intensity for ConA and PNA in the control group, suggesting that CP could increase the quantity of the receptor glycoproteins in the neural epithelium; thus interfering with the normal process of cell proliferation, migration, adhesion and recognition, and causing neural tube defects. Stainings in the treated group was significantly weaker than that in the control group, suggesting that the folic acid and vitamin C could inhibit the abnormal increase of the related receptor glycoproteins. and protect the normal process of cell proliferation and imigration from the interference of CP. which might be another important way for folic acid and vitramin C to prevent neural tube defects.