胎膜早破时母血和羊水中白细胞介素8和肿瘤坏死因子α的监测

Detecting cytokines in patients with preterm premature rupture of membrane

目的探讨胎膜早破及其绒毛羊膜炎孕妇血和羊水中细胞因子的变化。方法采用放射免疫法和酶联免疫吸附试验对４６例胎膜早破孕妇和２０例正常足月孕妇血及羊水中白细胞介素８（ＩＬ８）、肿瘤坏死因子（ＴＮＦ）α水平进行测定。结果胎膜早破孕妇血中ＩＬ８和羊水中ＩＬ８、ＴＮＦα水平均明显高于对照组（Ｐ＜０．０１～０．０５）；破膜时间与母血中ＩＬ８、羊水中ＩＬ８、ＴＮＦα水平有明显关系，破膜时间越长母血中ＩＬ８、羊水中ＩＬ８、ＴＮＦα水平越高；绒毛羊膜炎孕妇血和羊水中ＩＬ８、ＴＮＦα水平均明显高于非绒毛羊膜炎孕妇（Ｐ＜０．０１～０．０５）。结论母血和羊水中ＩＬ８、ＴＮＦα水平可以作为识别胎膜早破和绒毛羊膜炎的一个标志。

Objective To study the relationship of maternal blood and amniotic fluid cytokine levels in patients with preterm premature rupture of membrane and chorioamnionitis. Methods IL8 and TNFα levels of maternal blood and amniotic fluid were determined by 125 I radioimmunoassay (RIA) and an enzyme labeled immunosorbent assay (ELISA). Chorioamnionitis was diagnosed by fetal membrane histology.Results The maternal serum IL8 levels and amniotic fluid IL8, TNFα levels were higher than those of controls ( P <0.05). There was significant relationship between maternal serum and amniotic fluid IL8, TNFα with the time of the premature rupture of membanes. The longer the time the higher the maternal serum IL8 and amniotic fluid IL8, TNFα; There were 13 patients with chorioamnionitis, and their maternal serum and amniotic fluid IL8, TNFα were higher than those of patients with non chorioamnionitis ( P <0.01～0.05).Conclusion IL8, TNFα levels of maternal serum and amniotic fluid are valuble clinical indexes in identification of chorioamnionitis in patients with preterm premature rupture of membranes.