聚合酶链反应检测HGV RNA方法的建立及应用

DEVELOPMENT AND APPLICATION OF POLYMERASE CHAINREACTION METHOD FOR DETECTION OF GB VIRUS C/HEPATITIS G VIRUS

目的：了解我国不同人群中庚型肝炎病毒（ＧＢＶＣ／ＨＧＶ）感染的状况。方法：以ＮＳ５区序列分析为基础设计引物，建立聚合酶链反应方法，并对我国几个地区、不同疾病状况的２６８例患者进行庚型肝炎病毒核酸的检测。结果：有输血史的健康人群、ＨＢＶ和ＨＣＶ感染者及慢性非乙非丙型肝炎患者的ＧＢＶＣ／ＨＧＶＲＮＡ阳性率均高于自然人群，分别为１３．３％，１８．４％，１９．８％，８．９％，其中有明确血液暴露史者的检出率达２０．１％。结论：庚型肝炎在我国有广泛的流行，输血等肠道外途径为重要危险因素。

Objective: To investigate the infection of GB virus C/hepatitis G virus (GBVC/HGV) in Chinese population. Methods: We established a detection system using reverse transcriptase polymerase chain reaction (RTPCR) on the basis of sequence analysis of the nonstructural gene 5 (NS5) region of GBVC/HGV genome, which was applied in detection of viral RNA in 268 patients from different geographic areas of our country and with different liver diseases. Results: The positive rates of GBVC/HGV RNA in healthy people with history of transfusion, patients with HBV or HCV infection and chronic nonB, nonC hepatitis were higher than that of natural population (13.3%, 18.4%, 19.8% and 8.9% respectively). Among these patients, the group with history of blood exposure had high prevalence of infection (20.1%). Conclusion: GBVC/HGV infection is widespread in China, and transfusion is the main risk factor.