摘要
目的:应用双向单克隆抗体为桥梁对接种于雌性裸鼠体内的人乳腺癌细胞MCF7进行示踪,为示踪体内肿瘤细胞寻找更好的途径。方法:将MCF7细胞经体外培养后接种于雌性裸鼠,待形成肿瘤结节再用自制的双向单克隆抗体(用两株分别分泌抗MCF7细胞表面抗原及抗碘化的BoltonHunter,IBH试剂的单克隆抗体的杂交瘤再杂交,得到的双重杂交瘤分泌的双向单克隆抗体可同时结合MCF7细胞及IBH试剂)及125IBH注射于裸鼠体内,用放射自显影法显示125I在MCF7细胞表面的分布。结果:双向单克隆抗体确能将125IBH带到裸鼠体内的MCF7细胞表面。结论:应用双向单克隆抗体为桥梁进行肿瘤细胞示踪或局部放射治疗应是较单纯化学修饰单克隆抗体效果更加确切的方式。
Objective:To find out a better way to trace tumour cells in vivo by using a bispecific monoclonal antibody as the bridge. Methods: Cultured human mammary carcinoma cell line MCF7 cells were inoculated into female nude mice subcutaneously. After tumour nodule formed, a bispecific monoclonal antibody, (against both MCF7 cell surface antigen and a hapteniodinated BoltonHunter reagent, IBH, secreted by a quadridoma hybridized by two hybridomas, each parent hybridoma secreted one monoclonal antibody, against either MCF7 cell surface antigen or IBH), and125IBH were injected peritoneally 4 hours before sacrifice. Autoradiography was conducted to show if125IBH was bound to the surface of MCF7 cells. Results:125IBH was able to bind to the surface of MCF7 cells when the bispecific monoclonal antibody was employed as the bridge. Conclusion: Using the bispecific monoclonal antibody to trace tumour cells in vivo and /or perform local radiotherapy is more efficient compared with the way of chemical conjugation of a monoclonal antibody.
出处
《北京医科大学学报》
CSCD
1998年第1期21-23,共3页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
国家教委留学人员回国启动基金
关键词
乳腺癌
肿瘤循环细胞
单克隆抗体
放射性示踪剂
Antibodies, monoclonal Breast neoplasms Neoplasm circulating cellsRadioactive tracers